Whatman Westran™ PVDF (Polyvinylidine Fluoride) membranes are available in two formats: the Westran S used for protein sequencing and the Westran Clear Signal used for Western blotting
Westran S
Westran S PVDF is a 0.2 µm pore size hydrophobic membrane designed specifically for protein sequencing applications. The small pore size of this membrane eliminates ‘blow-through’ and increases protein binding over a wide range of molecular weights.
Features and benefits
- Protein binding capacity (over 200 µg/cm2) for easy signal detection
- Chemical resistance needed for N-terminal sequencing
- High protein retention even after harsh wash steps
- Maximum capture of proteins during transfers minimizing sample loss
- 0.2 µm pore size for higher surface area, resulting in better binding of low molecular weight proteins
- Compatible for use with Western blotting applications
- Available in popular precut sizes for your application
Westran clear signal
Westran Clear Signal (CS) PVDF is a 0.45 µm membrane specifically designed for Western blotting and protein dot-blotting applications.
Features and benefits
- Protein binding capacity over 125 µg/cm2
- Extremely low backgrounds with chemiluminescent and colorimetric applications providing you with clear signals and sharp bands
- Excellent results with general protein stains such as Coomassie® Brilliant Blue, Amido Black, and Ponceau S Red
- Increased strength allows for multiple stripping and reprobing, which results in savings
Westran blotting sandwiches
A precut PVDF membrane and 2 sheets of 3MM Chr blotting paper are prepackaged into a sandwich to help save time. Sandwiches are available with either Westran S or CS.