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UniSep High Performance Liquid Chromatography (HPLC) Columns
Home | Chromatography Products | High Performance Liquid Chromatography (HPLC) | UniSep High Performance Liquid Chromatography (HPLC) Columns

The UniSep HPLC column is the newest C-8 reverse phase HPLC column from Whatman. Using state-of-the-art technology, UniSep was developed for conditions that call for a highly aqueous mobile phase.

The advantage of the UniSep C-8 column over a traditional C-8 column is the UniSep silica is hydrophilic, or able to be wetted out. This change in hydrophobicity is achieved by attaching an ether linkage in close proximity to the silica backbone. Since the ether group is polar, water can easily penetrate and hydrate the silica surface, allowing the analyte greater access to the binding sites. The advantage to the chromatographer is the flexibility gained when developing a method for highly water soluble compounds.

Features and benefits
  • C-8 reverse phase
  • Hydrophilic silica surface
  • 5 µm particle size
  • 100Å pore size
  • 16% carbon load
  • Easy scale up
  • WVS format

Applications
  • Life science
  • Food and beverage
  • Pharmaceutical

This and other media are described in the HPLC media section.
Guard Cartridges are available to prolong analytical column life.

Note: Use of WVS columns requires a one-time purchase of WVS end fittings (catalog number 4631-1001).


Ordering Information - UniSep High Performance Liquid Chromatography (HPLC) Columns
Catalog Number Description

UniSep 5 µm Reverse Phase HPLC Columns

4631-1001 Reusable WVS end fittings, 1 pair/pk

Supporting Documentation - UniSep High Performance Liquid Chromatography (HPLC) Columns
Document Name
HPLC Quick Pick Reference Chart
Support Documentation
Ordering Information
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