Using leading-edge, automated microarray technologies and innovative scientific approaches, the protein array development, processing, and data analysis service allows researchers to acquire distinct, reliable scientific data from the proteomic specialists at Whatman. Protein array development, within our facility, encompasses proof-of-principle, assay design, array design, and printing, processing and data analysis.
Quantitative Cytokine Array Processing and Data Analysis
Based upon the FAST Quant System, the quantitative cytokine array processing and data analysis service offers researchers the results from the quantitative analysis of multiple cytokines processed in a FAST Slide based, micro ELISA format. Signal output from FAST Quant arrays is detected with a standard fluorescent microarray scanner and data is analyzed using the ArrayVision FAST software
- Select from the Whatman antibody menu of 40 human and 19 murine specificities
- Preserve valuable samples – high-throughput ELISA format requires as little as 140 µl of human sample per array
- Applicable sample types include serum, plasma, cell culture supernatants, cellular extracts, and wound effluent
- Reduces costs associated with the cost of antibodies, capital equipment, assay development, lab personnel, software acquisition, and analysis
- Processed and analyzed by expert staff in dedicated array processing facility
The following FAST Quant data and images are uploaded to the FTP site:
- Total fluorescent signal per microspot
- Standard curves per cytokine
- % CV
- Standard deviation
- pg/ml value per sample
Comparative Analysis of Known Serum Biomarkers
The Serum Biomarker sample processing service makes it simple for researchers to outsource the tests or try this new technology before adopting it in their labs.
- Customer samples sent to Whatman and processed using the Serum Biomarker Chip
- Data uploaded to a password-protected FTP site within ten business days of receipt of samples at Whatman
- Minimum order: two paired serum samples
The following Serum Biomarker Chip processing data and images are uploaded to the FTP site:
- Total fluorescent signal per microspot
- % CV
- Standard deviation
- Graphed histogram of ratios
- Average of signal-background triplicate microspots
- Z score to identify outlier microspots that are significantly different from the mean
See Specificities list in Serum Biomarker Chip section