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Whatman protein array reagents have been optimized for use on FAST Slides. These reagents include Protein Arraying Buffer, Protein Array Blocking Buffer and Protein Array Wash Buffer.
Protein Arraying Buffer
- Enhances long-term protein stability and molecular recognition activity
- Enhances fluorescent signal from arrayed sample
- Optimized for use on FAST Slides
Protein Array Blocking Buffer
- Demonstrates highly efficient blocking of protein microarrays
- Exhibits strong reduction of non-specific antibody-antibody interactions
- Exhibits minimal effects on specific antibody-antigen interactions
- Results in superior signal-to-noise ratios in protein microarray applications
- Superior blocking capabilities
Reduction of non-specific protein-protein interaction:
Monoclonal capture antibodies were arrayed on FAST Slides and probed with an antibody cocktail consisting of 16 biotinylated polyclonal antibodies followed by Streptavidin-Cy5 detection. Images were taken at an identical laser/PMT setting. The first row of the array field is a positive detection control.
Blocking with Protein Array Blocking Buffer:
Reduction of non-specific antibody-antibody interactions by the protein array blocking buffer.
Protein Array Wash Buffer
- Ideal washing buffer for protein microarrays
- Conveniently supplied as 10x concentrate
- Optimized for use on FAST Slide
The Protein Array Wash Buffer has been optimized for efficient washing of protein microarrays to ensure optimum results. This buffer is used as the washing buffer in FAST PAK, FAST Quant and the Serum Biomarker Chip kits.
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