Plasmid/BAC Sample Preparation

96 Well Bacterial Growth Plate
The Whatman high-throughput Bacterial Growth Plate can simplify and accelerate the growth of 96 individual 1.5 mL bacterial cultures. It is used for both overnight cultivation and the initial ‘spin down’ of bacteria. Made of medical grade polypropylene with a clear polystyrene lid, this gamma-irradiated plate eliminates the need to grow multiple, discrete cultures. It also optimizes space and efficiency in the incubator.

96 Well Lysate Clarification UNIFILTER^®
The Whatman Lysate Clarification UNIFILTER can utilize either vacuum or a centrifuge. The vacuum process is significantly easier to automate with consistency across all wells. It also has an average DNA recovery rate 10-30% higher than the manual centrifuge method. This method filters out cell debris to obtain plasmid DNA in the aqueous phase. Whatman filter technology results in high particle retention and fast flow rates while producing a clean lysate. The Lysate Clarification plate is an important tool for high-throughput plasmid DNA purification.

96 Well DNA Binding UNIFILTER
Whatman Plasmid DNA Binding UNIFILTER works either as a stand-alone plate or as part of our high-throughput miniprep system. Plasmid DNA is bound to the filter under chaotropic conditions, washed twice and then vacuumed to dryness on a vacuum manifold. The plasmid DNA is eluted by vacuum in a final volume of 100 µL into a non-binding polypropylene collection plate using water or TE^-1 buffer. The DNA is ready to use and further ethanol precipitation is unnecessary. The final concentration is 50-100 ng/µL, depending on the original culture. The OD260/280 ratio is 1.9 and the yields in all 96 wells ‘max out’ at 6 µg.

The Plasmid DNA Binding plate can be used with both vacuum and centrifuge techniques, making it a vital and flexible tool in every high-throughput laboratory.

384 Well DNA Binding UNIFILTER
The 384 Well DNA Binding UNIFILTER plate effectively binds and purifies DNA molecules. It provides highly reproducible results with yields exceeding 2 µg/well, from bind-wash-elute processing with collection by filtration. Minimal liquid hang-up allows for reduced elution volume, enabling DNA concentration as high as 150 ng/µL. Further ethanol precipitation is unnecessary. The DNA is ready to use.

BAC Prep UNIFILTER
With increasing demand for simple and fast methods to purify DNA from bacterial cultures, the Whatman BAC Prep microplate is the ideal solution for the clarification of lysates containing large insert vectors.

This microplate has a cellulose acetate membrane with a special support, which clears non-chaotropic bacterial lysates, and long drip directors. Without further purification, the DNA is clean enough for further enzymatic manipulation. Cellulose acetate acts as both a depth filter and a fine particle filter. The 0.45 µm pores do not block because of the depth effect of the membrane. Cellulose acetate is also inert and does not bind either DNA or protein.

Ordering Information - Plasmid/BAC Sample Preparation

Catalog Number

Description

96 Well Bacteria Growth Plate

7701-5205

UNIPLATE, 96 wells, 2 mL, Natural Polypropylene, Round Well Bottom, Irradiated with Lid, Individually bagged 25/pk

96 Well Lysate Clarification UNIFILTER

7720-2830

Lysate Clarification Plate, 96 wells, 800 µL, Clear Polystyrene 25/pk

96 and 384 Well DNA Binding UNIFILTER

7700-2810

UNIFILTER, 96 wells, 800 µL, DNA Binding, Clear Polystyrene, Filter Bottom with Long Drip Director 25/pk