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Normal Phase (Adsorption) Media
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| Partisil Silica (5 and 10 µm) |
| Pure silica stationary phase for adsorption chromatography. Partisil 10 is used particularly for routine separations for higher flow rates and lower back pressures. Partisil 5 is used particularly for higher resolution and fast analysis. These are the supports on which the Partisil bonded phases are based. |
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| PartiSphere Spherical Silica (5 µm) |
| PartiSphere silica features homogenous particles with narrow particle size distribution for sharp separations and excellent reproducibility. It is the basis for PartiSphere bonded phases. |
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Ion Exchange Media
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| Partisil SAX (5 and 10 µm) |
| A strong anion exchanger based on quaternary ammonium groups (-NR+3 ). Supplied in the H2PO4 form in methanol, Partisil 10 SAX has been widely reported in literature and is best known for the separation of nucleotides. Stable over pH range 1.5-7.5 when used in conjunction with a Solvecon mobile phase conditioning column. Obtains the highest anion exchange efficiencies and resolution. Applicable to separations of nucleic acids, organic acids and inorganic anions. |
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| Partisil SCX (5 and 10 µm) |
| Based on aromatic benzene sulfonic acid groups. Supplied in the ammonium form. Excellent for separation of nucleosides, amino acids, polyamines, drugs and other cationic species. Capable of being loaded with specific metallic cations for use in ligand exchange chromatography. Stable over pH range 1.5-7.0 when used in conjunction with a Solvecon mobile phase conditioning-column. Exceptionally stable Si-O-Si-C bond, both thermally and chemically. |
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| PartiSphere SAX and SCX (5 µm) |
| Strong ion exchange media based on homogenous spherical silica particles with very tight size distribution. They produce very sharp separations. |
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| Partisil and PartiSphere PAC |
| Polar Amino Cyano weak anion exchanger -- described below. |
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Reverse Phase Media
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| Partisil ODS (10 µm) |
| A C-18 phase with a 5% carbon load for both normal adsorption and reversed phase partitioning. Dual-mode operation for added selectivity with 50% residual silanols. Lightly loaded C-18 packing is particularly effective for compounds having greater water solubility when used in the reversed phase mode. Creates a moderately polar surface, different from that of pure silica, in normal phase mode. |
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| Partisil ODS-2 (10 µm) |
| The high carbon load (16%) of this polymeric phase makes it the most nonpolar and, therefore, the most retentive of the reversed phases. An alternative to end-capped C-18 where different elution order is desirable for optimum separation. High sample load capacity and 10 µm particle size are very suitable for preparative work. |
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| Partisil ODS-3 (5 and 10 µm) |
| A C-18 polymeric phase with a 10.5% carbon load. Medium of choice for improved speed, efficiency and resolution in applications requiring C-18 phases. End-capped for deactivation of silanols to minimize the need for ion suppression or ion pairing agents. Used in a wide range of applications with optimal selectivity, including pharmaceuticals, natural products, food, biological and environmental pollutants. |
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| PartiSphere C-18 (5 µm) |
| C-18 stationary phase on homogenous, spherical silica particles for very high separation efficiency. |
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| PartiSphere RTF C-18 (5 µm) |
PartiSphere RTF (Reduced Tailing Factor) C-18 is base-deactivated . It employs a unique proprietary process that effectively ‘deactivates’ the secondary chromatographic effect due to residual silanols. In addition, this material is extremely stable and can be used from pH 2 to pH 8 with no loss in performance. Excellent for separation of basic compounds without the need for amine-modified mobile phases.
One type has a polar linker similar but not identical to the UniSep C-8 meda. |
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| Partisil C8 (5 and 10 µm) |
| An end-capped C-8 monomeric phase with at least 8.5% carbon load. Provides high efficiency and rapid mass transfer while maintaining excellent peak shape and stability over a range of aqueous mobile phase compositions. Recommended for ion pair chromatography. |
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| PartiSphere C-8 (5 µm) |
| Octyl bonded phase on homogenous spherical particles for maximal resolution. |
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| UniSep C-8 (5 µm) |
| Hydrophobic octyl chain on hydrophilic silica surfae, enabling the silica to be wetted out. Ideal for highly aqueous mobile phases. |
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| Partisil PAC (5 and 10 µm) |
| A polar amino cyano bonded phase with secondary amine groups for good thermal and chemical stability. Selectivity and rapid equilibrium allow a range of separation mechanisms to be used, including adsorption, reversed phase and weak anion exchange. Extremely fast equilibration across the entire range of solvents from heptane to water. The media of choice for carbohydrate separations. |
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| PartiSphere PAC (5 µm) |
| Polar Amino Cyano bonded to homogenous spherical particles for maximal resolution. |
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Application-Specific Media
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| TAC 1 (5 µm) |
| For great discoveries such as Taxol, Whatman technology optimally separates the closely eluting taxanes of Pacific yew trees. Whatman worked closely with two leading customers to develop a specific bonded phase that achieves baseline resolution of the paclitaxel molecule from its closest impurity. Each lot of TAC 1 (Taxane Analysis Column) is tested with a paclitaxel chromatographic purity separation to ensure the best possible reproducibility. (Richheimer SL et al. Anal Chem. 1992; 64: 2323-2326) |
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| MAX-1 (5 µm) |
| For use in specialized separation for corn and soy protein. |
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