The GenSpin Plant DNA Purification Kit is designed for the rapid preparation of double-stranded DNA in solution from small quantities of plant material for PCR analysis.

Using a single micro centrifuge tube, this simple protocol enables the recovery of DNA for more than 50 amplification reactions from just 10 mg of plant tissue. The small sample capability is ideally suited for rapid analysis studies such as identification of genetically modified plants and cultivar screening.

Plant material is homogenized at room temperature and applied to the GenSpin Filter Basket, which incorporates FTA technology to immediately stabilize the DNA at room temperature. Nucleases are inactivated and the DNA is protected from UV and environmental damage. The immobilized DNA is entrapped in the fibers of the matrix and can either be purified immediately or stored at room temperature for more than four weeks. The filter is washed with two reagents to remove contaminants that would inhibit PCR. The DNA is then eluted from the filter by heating and collected by centrifugation.

• Simple, single tube protocol. Eliminates need for organic solvents, liquid nitrogen and time-consuming precipitation steps.
• Fast purification of DNA. Purify DNA in less than 30 minutes for quick sample screening. Up to 50 amplifications from only 10 mg of plant material.
• PCR-ready double-stranded DNA. Reliable amplification of DNA for a wide range of applications including cultivar screening and identification of genetically modified plants.
• FTA technology protects DNA from degradation. Enables room temperature storage for weeks.

*Manual method (Dellaporta et. al. 1983) does not include time required for full resuspension after DNA precipitation Reference: Dellaporta et al. (1983) A plant DNA minipreparation. Plant Molecular Biology Reporter 1:19-21

*Extraction of these plant species requires the addition of DTT to Homogenization Buffer.  DNA yields can vary depending on plant species, tissue age and growing conditions.  Double-stranded DNA was quantified using PicoGreen™ Reagent (Invitrogen)