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FAST Quant 40 HumanTM
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Home | Protein Microarrays | Pre-Arrayed FAST Slides | FAST Quant 40 HumanTM
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New FAST Quant 40 HumanTM Kit lets you measure concentrations of 40 human cytokines at once!
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| Angiogenin |
IGF-I |
IL-12p40 |
PDGF-BB |
| Angiopoietin-2 |
IGF-II |
IL-12p70 |
RANTES |
| CRP |
IL-1alpha |
IL-13 |
TGF-alpha |
| Eotaxin |
IL-1beta |
IP-10 |
TGF-beta1 |
| FAS ligand |
IL-2 |
KGF |
TGF-beta3 |
| FGF-basic |
IL-4 |
MCP-1 |
TIMP-1 |
| Fibronectin |
IL-5 |
MCP-3 |
TNF-alpha |
| GM-CSF |
IL-6 |
MIP-1alpha |
TNFR-I |
| ICAM-1 |
IL-8 |
MMP-7 |
TNFR-II |
| IFN-gamma |
IL-10 |
MMP-9 |
VEGF |
FAST Quant Kits combine carefully-tested, sensitive immunoassays with Whatman FASTTM Slides, the surface of choice for protein microarrays. Previous kits allow measurement of concentrations of 8 - 10 human or mouse cytokines in one assay. This new kit combines the 40 most-requested human cytokines in one kit, allowing you to collect data and analyze relationships that were impractical to explore before.
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MicroSpot ELISA for High-Throughput Multiplex Cytokine Quantification
FAST Quant represents a quantum leap forward in protein microarray technology. With FAST Quant, a researcher can accurately determine the concentration of forty human cytokines in 24 biological samples simultaneously, using familiar ELISA immunochemistry and fluorescent detection.
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FAST Quant 40 Human Kit
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Built on FAST technology, the high protein binding capacity surface chemistry, FAST Quant combines the power of array technology with the quantitative nature and high-throughput capabilities of traditional ELISA. FAST Quant exhibits sensitivity and reproducibility better than traditional ELISA.
A standard curve is generated by creating a dilutional series from recombinant antigen mass standards. Due to the solid-phase nature of a microspot assay, it is not necessary to take duplicate measurements of each sample. The MicroSpot is entirely concentration dependent, unlike an ELISA where the reaction is both concentration and volume dependent.
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FAST Frame with
FAST Slides and
Incubation Chambers
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| The 40 analytes are divided into 2 sub-arrays of individual cytokine-specific antibodies, spotted in triplicate. Each slide contains 8 pads arrayed with Sub-array A and 8 pads with Sub-array B. The kit of 4 slides, used with a FAST Frame holder (ordered separately) gives standard curves for each Sub-Array plus full cytokine concentration measurement on 24 samples. The FAST Frame provides an 8x12 cm footprint for use of standard multiwell pipettors and liquid handling devices. |
| Data analysis uses MicroVigene™ for FAST Quant data reduction software, the cutting edge utility for protein array image analysis. Adaptation to the 2-sub-array format of the new kit is underway. Until a customized version becomes available customers will send scanned .tif files to Whatman by FTP for data analysis. |
| FAST Quant 40 Human is another clear example of Whatman commitment to providing the scientific community with the best solution for multiplex cytokine measurements. |
For research use only. |
Sub-array A Analytes, Limits of Detection and Ranges:
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Analyte
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LOD (pg/ml)
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Range (pg/ml)
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Analyte
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LOD (pg/ml)
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Range (pg/ml)
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IL-1alpha
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1
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1-1000
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IL-13
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50
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50-25000
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IL-1beta
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1
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1-2000
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IFN-gamma
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20
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20-25000
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IL-2
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1
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1-3000
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TNF-alpha
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1
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1-1000
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IL-4
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1
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1-6000
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MMP-9
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3
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3-6000
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IL-5
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1
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1-3000
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MCP-1
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1
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1-2000
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IL-6
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1
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1-2000
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IGF-II
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2000
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2000-300000
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IL-8
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1
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1-1000
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TNFR-I
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2
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2-3000
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IL-10
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50
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50-50000
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TGFalpha
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1
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1-600
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IL-12p70
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20
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20-20000
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TGFbeta3
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1
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1-2000
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Sub-array B Analytes, Limits of Detection and Ranges:
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Analyte
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LOD (pg/ml)
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Range (pg/ml)
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Analyte
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LOD (pg/ml)
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Range (pg/ml)
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Angiogenin
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5
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5-20000
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FGF basic
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60
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60-60000
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Angiopoietin-2
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50
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50-50000
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FAS ligand
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1
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1-6000
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Eotaxin
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4
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4-3000
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MCP-3
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5
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5-2000
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ICAM-1
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5
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5-30000
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Fibronectin
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10
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10-20000
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IGF-I
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10
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10-50000
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PDGF-BB
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15
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15-50000
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MMP-7
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3
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3-6000
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TGF beta1
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9
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9-20000
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KGF
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5
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5-10000
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TIMP-1
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9
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9-30000
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IP-10
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5
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5-6000
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TNFR-II
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10
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10-30000
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GM-CSF
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1
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1-2000
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VEGF
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2
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2-10000
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IL-12p40
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30
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30-50000
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RANTES
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2
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2-2000
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CRP
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1
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1-1000
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MIP-1alpha
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5
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5-10000
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Each kit contains 4 arrayed 16-pad slides (each with 8 pads of Sub-array A and 8 of B), 4 Incubation Chambers, Blocking Buffer, Washing Buffer, biotinylated detection antibody solutions for each sub-array, recombinant antigen solutions for each sub-array, Streptavidin-Cy 5 for fluorescent detection, an array template and a protocol booklet.
The FAST Frame Slide Holder #10486001 is available separately.
Please see the main FAST Quant page for other kits in this series.
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