High Recovery of DNA
The Elutip-d minicolumns are designed for purification of DNA with high recovery rates. They provide a simple and convenient method for purification of DNA in the15 bp to 50 Kb range.
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The Elutip-d columns are ideal for removal of unincorporated nucleotides and other contaminants from radiolabeling reactions to reduce background levels and increase sample activity. They also provide an excellent method for isolation of nucleic acids from low-melt agarose gels.
The Elutip-d column matrix binds nucleic acids in high quantities upon sample application under low salt conditions. Contaminants are washed through the column and the purified DNA is then eluted with high salt. The eluted sample is ready for use in a variety of assays that require high purity of the nucleic acid.
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Elutip-d
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Elutip-d columns are used with standard syringes. Optional prefilters contain non-binding cellulose acetate membranes and are designed to increase efficiency by removing gel pieces that could otherwise clog the column.
Features and Benefits
- High recovery of single- and double-stranded DNA
- Eliminates contaminants that can cause high background or interfere with sample activity
- Sample is eluted in a small volume
- 100 µg capacity