EXPRESS-ION™ media are matrices whose flow characteristics have been greatly improved. The manufacturing process has been optimized so that the matrix allows faster flow while retaining its inherently fast kinetics of adsorption and desorption. They are supplied as moist powders, so precycling and fines removal are not required.
EXPRESS-ION D
A weak (charge dependent on pH) anion exchange cellulose substituted with diethylaminoethyl (DEAE) groups and recommended for separations between pH 2 and pH 9.
EXPRESS-ION Q
A strong (charge independent of pH) anion exchange cellulose having general applicability in separations requiring an anion exchange step, with the benefit of wide pH versatility. The N,N,N-trimethyl hydroxypropyl amine (quaternary amine) group is fully ionized throughout pH range 2 to 12.
EXPRESS-ION C
A moderately acidic cation exchange cellulose. A weak ion exchanger recommended for separations between pH 4.5 and pH 10 to ensure the carboxymethyl functional group remains ionized.
EXPRESS-ION S
A strongly acidic cation exchange cellulose having general applicability in separations requiring a cation exchange step, with the benefit of wide pH versatility. The sulfoxyethyl functional group is fully ionized throughout the pH range 2 to 12.
Standard Anion and Cation Exchange Media are described separately.
Ion Exchange Celluloses are also available in paper format.
| Typical Data - EXPRESS-ION linear flow rate (cm/h) |
| |
Linear flow rate (cm/h) |
| |
at 5 psi |
at 7.5 psi |
at 10 psi |
| EXPRESS-ION D |
172 |
214 |
239 |
| EXPRESS-ION Q |
135 |
174 |
212 |
| EXPRESS-ION C |
94 |
127 |
165 |
| EXPRESS-ION S |
94 |
127 |
161 |
| Column dimensions 45 cm ID x 15 cm H |
| Properties - EXPRESS-ION High Flow Rate Media |
| |
EXPRESS-ION D |
EXPRESS-ION Q |
EXPRESS-ION C |
EXPRESS-ION S |
| Type |
Weak Anion |
Strong Anion |
Weak Cation |
Strong Cation |
| Working pH range |
2 to 9 |
2 to 12 |
4.5 to 10 |
2 to 12 |
| Typical protein capacity mg/ml: |
|
|
|
|
| BSA |
60 |
55 |
- |
- |
| Lysozyme |
- |
- |
162 |
153 |
| Small ion capacity |
1 meq/dg* |
| Fiber length |
60 to 130 µm |
| Base matrix |
Microgranular cellulose |
| Typical flow rate |
150 cm/hr |
| Physical stability |
Negligible volume changes due to ionic strength or pH |
| Bed volume g† / ml |
0.72 |
0.64 |
0.7 |
0.8 |
* dg = dry gram, Typical moisture content 65 to 68%
† grams as supplied |