Creating an antibody-based protein microarray
The custom array service at Whatman enables scientists who have access to microarray scanning and data analysis instrumentation to have FAST Slides printed with proteins from the Whatman antibody menu or from their own protein library.

The custom service provides the scientist complete control of proof-of-principle, assay design, processing, and data analysis of the printed array.

Customer-supplied biological samples are screened against the selected specificities to determine the quantitative or qualitative measurements, depending upon the customer’s requirements. The deliverables to the researcher include access to raw and trimmed data, for each analyte, total fluorescent signal per microspot (quantitative assays only), % CV and standard deviation, and graphed histograms of ratios, signal-background averages, and outlier recognition.

Protein array development projects are driven by certified R&D project leaders responsible for the scope, resources, and timeline that meet the needs of the researcher. Project implementation, project milestones, and customer communications are managed by dedicated account specialists, in collaboration with the research staff.

Protein specificities, whether they are from Whatman or customer supplied, are arrayed to the selected FAST Slide configuration – using a quill-pin contact or noncontact printing technology – in triplicate, unless otherwise specified – suspended in Whatman protein arraying buffer to achieve long-term stability.

Creating an antibody-based protein microarray

Step 1: Are you going to print the array yourself? If yes, look to our line of FAST Slide products and array buffers to ensure outstanding results.

Step 2: Would you like to array from our available menu? If yes, simply contact us with details of the required specificities and arrays. Our experts will define the best adapted configuration to your specific arrays. Our menu is constantly expanding and we are happy to accommodate specific requests. Select the proteins you need to have printed from the antibody menu or send your own library of proteins. Receive your custom arrayed FAST Slides within 15 to 20 business days.

Step 3: If you would like us to process your samples on a custom or off-the-shelf array simply contact us and provide details of the number and type of samples required.