Membrane-based Antibody Arrays
Whatman FAST Macro membrane-based antibody arrays are used to simultaneously evaluate the relative abundance of 20 different cytokines between different biological samples, such as disease state versus normal state, using chemiluminescent detection.

The FAST Macro Human I and Mouse I arrays consist of 20 anti-cytokine antibodies arrayed on Whatman Protran nitrocellulose (BA83, 0.2 µm), a surface which is well known for high protein binding capacity and excellent signal-to-noise ratios.

FAST Macro Membrane-based Antibody Array

Designed as a screening tool to compare relative expression levels of cytokines/growth factors in different samples, one human panel and one mouse panel are included. The FAST Macro Kit includes either four or eight arrayed membranes, FAST Macro Wash and Blocking Buffers and a biotinylated antibody cocktail. Arrays are visualized* using streptavidin/HRP- based chemiluminescent detection and x-ray film or a phosphor imager.

FAST Macro is an inexpensive way to discover the power of multiplex antibody arrays with sensitivity as low as 15 pg/mL that can be used with serum, cell lysates and culture media samples. Results are comparable to ELISA results. No special instrumentation or software is required for detection and analysis.

* Detection reagents must be provided by the end-user. See recommendations at www.arraying.com

Features
• Requires as little as 650 µL of sample
• Array-to-array reproducibility
• Cytokines are arrayed in triplicate along with 9 positive and 3 negative control spots per membrane
• Tested for cytokine specificity – virtually no cross reactivity
• Membrane is notched in one corner for orientation
• Cytokine spots are visible on the unprocessed membrane – inert dye disappears during membrane wet-out

 FAST Macro Kit