Blotting Membrane Selection Guide
	Protran	Optitran	Nytran N	Nytran SuPerCharge	Westran S	Westran Clear Signal
MEMBRANE TYPE:	Nitrocellulose, 100% pure	Nitrocellulose, reinforced	Nylon, moderately positively charged	Nylon, highly positively charged	PVDF	PVDF
APPLICATIONS:	Western, Southern, Northern blotting...	Western, Southern, Northern blotting...	Southern, Northern blotting...	Southern, Northern blotting...	Western blotting... Sequencing	Western blotting...
BINDING	75-110 µg/cm2	75-90 µg/cm2	>400 µg/cm2	>600 µg/cm2	>50-100 µg/cm2	>50-100 µg/cm2
PORE SIZES:	0.45 µm 0.2 µm 0.1 µm	0.45 µm 0.2 µm -	0.45 µm 0.2 µm -	0.45 µm - -	- 0.2 µm -	0.45 µm - -
TRANSFER METHODS:
Semi-dry Blotting	++	++	++	++	++	++
Tank Blotting	++	++	++	++	++	++
Vacuum Blotting	++	++	++	++	+	+
Capillary Blotting	++	++	++	++	+	+
Alkaline Method	not recommended	not recommended	++	++	not recommended	not recommended
IMMOBILIZATION:
UV-crosslinking, DNA, RNA	++	++	++	++	-	-
Baking (80° C), DNA, RNA	++	++	+	+	-	-
Drying, DNA, RNA	-	-	+	+	-	-
Drying, Protein	++	++	-	-	++	++
DETECTION METHODS:
Colorimetric	++	++	+	+	++	++
Chemiluminescent	++	++	++	++	++	++
Isotopic	++	++	++	++	++	++
Fluorescent	++	-	-	-	-	-
REPROBING:	limited	++	++	++	++	++
++  recommended   +  satisfactory