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Blotting Membranes
Home | Blotting Products | Blotting Membranes
Blotting Membrane Selection Guide        
  Protran Optitran Nytran N Nytran
SuPerCharge		 Westran S Westran
Clear Signal
Membrane type Nitrocellulose,
100% pure		 NNitrocellulose,
reinforced		 Nylon, moderately
positively charged		 Nylon, highly
positively charged		 PVDF PVDF
Applications Western,
Southern,
Northern blotting...		 Western,
Southern,
Northern blotting...		 Southern,
Northern blotting...		 Southern,
Northern blotting...		 Western
blotting...
Sequencing		 Western
blotting...
Binding 80 to 150
µg/cm2		 75 to 90
µg/cm2		 > 400
µg/cm2		 > 600
µg/cm2		 > 50 to 100
µg/cm2		 > 50 to 100
µg/cm2
Pore sizes 0.45 µm
0.2 µm
0.1 µm		 0.45 µm
0.2 µm
-		 0.45 µm
0.2 µm
-		 0.45 µm
-
-		 -
0.2 µm
-		 0.45 µm
-
-
Transfer methods:        
Semi-dry Blotting ++ ++ ++ ++ ++ ++
Tank Blotting ++ ++ ++ ++ ++ ++
Vacuum Blotting ++ ++ ++ ++ + +
Capillary Blotting ++ ++ ++ ++ + +
Alkaline Method not recommended not recommended ++ ++ not recommended not recommended
Immobilization:        
UV-crosslinking, DNA, RNA ++ ++ ++ ++ - -
Baking (80°C), DNA, RNA ++ ++ + + - -
Drying, DNA, RNA - - + + - -
Drying, Protein ++ ++ - - ++ ++
Detection methods:         
Colorimetric ++ ++ + + ++ ++
Chemiluminescent ++ ++ ++ ++ ++ ++
Isotopic ++ ++ ++ ++ ++ ++
Fluorescent ++ - - - - -
Reprobing: limited ++ ++ ++ ++ ++
++  recommended
  +  satisfactory

 
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Protran Nitrocellulose Membranes
Optitran Nitrocellulose Membranes
Westran PVDF Membranes
Nytran Nylon Membranes
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