The DE weak (charge dependent on pH) anion exchangers are based on the diethylaminoethyl (DEAE) tertiary amine functional group. QA52 is a strong (charge independent of pH) anion exchange medium, containing quaternary amine groups.
DE23 dry fibrous DEAE cellulose
Allows fast flow rates especially after fines removal; suitable for negatively charged biopolymers.
DE32 dry microgranular DEAE cellulose
Similar performance characteristics after precycling as DE52.
DE52 preswollen microgranular DEAE cellulose
Probably the most widely used DEAE cellulose in the world; used for biopolymers with low to high negative charges; exhibits excellent resolution with good flow rates.
DE53 preswollen microgranular DEAE cellulose
Partially quaternized DEAE anion exchanger, highly substituted and with higher capacity than DE52; can be used in series with DE52 media.
QA52 preswollen microgranular QA cellulose
A strongly basic, quaternary amine-bearing anion exchange medium, moderately substituted, with high protein capacity. Fully ionized, bears constant charge under all pH conditions; excellent for high pH applications.
EXPRESS-ION High Flow Rate Ion Exchange Media are described separately.
Ion Exchange Celluloses are also available in paper format.
| Typical Data - Anion Exchange Media |
| Phsical form |
Functional group |
Normal pH
range |
Small ion capacity
(meq/dg*) |
Protein Capacity‡ |
Packing density
(g†/ml) |
| (mg/dg*) |
(mg/ml) |
| Dry Fibrous |
|
|
|
|
|
|
| DE23 |
Diethylaminoethyl |
2 to 9.5 |
0.88 to 1.08 |
425 |
60 |
0.15 |
| Dry Microgranular |
|
|
|
|
|
| DE32 |
Diethylaminoethyl |
2 to 9.5 |
0.88 to 1.08 |
700 |
140 |
0.24 |
| Preswollen Microgranular |
|
|
|
|
|
| DE52 |
Diethylaminoethyl |
2 to 9.5 |
0.88 to 1.08 |
700 |
130 |
0.9 |
| DE53 |
Diethylaminoethyl |
2 to 12 |
1.8 to 2.2 |
750 |
150 |
1.1 |
| QA52 |
Quaternary Ammonium |
2 to 12 |
1.1 |
750 |
150 |
1.2 |
* dg = dry gram;
† g = gram as supplied (dry or preswollen)
‡ Bovine serum albumin in 10 mM phosphate buffer pH 8.5 |