96 Well DNA Binding UNIFILTER

96 Plasmid DNA Binding UNIFILTER

Plasmid DNA Binding UNIFILTER works either as a stand-alone or as part of our high-throughput miniprep system.

Plasmid DNA is bound to the filter under chaotropic conditions, washed twice, and then vacuumed to dryness on a vacuum manifold. The plasmid DNA is eluted by vacuum in a final volume of 100 µl into a nonbinding polypropylene collection plate using water or TE^-1 buffer. The DNA is ready to use and further ethanol precipitation is unnecessary. The final concentration is 50 to 100 ng/µl, depending on the original culture. The OD260/280 ratio is 1.9 and the yields in all 96 wells 'max out' at 6 µg. Full protocol is available at www.whatman.com

The Plasmid DNA Binding plate can be used with both vacuum and centrifuge techniques, making it a vital and flexible tool in every high-throughput laboratory.

Features and benefits

DNA recovery of 6 µg per well on average
Consistent yield across all 96 wells
Eluted plasmid DNA is free of genomic DNA contamination when used with Lysate Clarification UNIFILTER
High quality DNA suitable for PCR, restriction digestion, and sequencing
Save time: no desalting or ethanol precipitation
No kit required, significantly reduces costs

Ordering Information - 96 Well DNA Binding UNIFILTER
Catalog Number	Description
7700-2810	UNIFILTER, 96 wells, 800 µl, DNA binding, clear polystyrene, filter bottom with long drip director, 25/pk

Supporting Documentation - 96 Well DNA Binding UNIFILTER

Document Name

High Throughput Plasmid DNA Binding Plate - High Throughput Plasmid DNA Binding Plate