The Whatman Single Color ULS™ Biotin Labeling Kit is designed to label serum or cell lysate proteins in order to detect their binding to complementary antibodies. The kit is especially intended for use with 2 pad FAST Slides, including the Serum Biomarker Chip and other single-capture antibody chips. The kit contains Universal Linkage System (ULS) chemistry from KREATECH® Biotechnology to label samples containing approximately 100 to 120 µg of protein in serum or plasma or 60 µg protein in a whole cell lysate.
Features
- Highly efficient and uniform labeling of complex protein samples
- Reproducible labeling and signal detection
- Stable, robust, and fast non enzymatic process
- Broad pH range of labeling
- Compatible with most buffers, salts, detergents, and reducing agents
- Labels multiple amino acids
ULS chemistry tags protein methionine, cysteine and histidine residues with biotin (normally at least two biotins per molecule). The attached biotin is then detected with fluorescent streptavidin.