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Whatman FTA® DMPK Cards
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Home | Whatman FTA DMPK Cards | Whatman FTA DMPK Cards | Whatman FTA® DMPK Cards
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New Products – Indicating FTA DMPK cards for use with colorless samples such as plasma, urine and CSF. (See below.)
Dried blood spot microvolume sampling for DMPK
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Overview
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| Drug metabolism (DM), pharmacokinetic (PK) and toxicokinetic (TK) studies provide crucial insight into how drug candidates behave in the body and are therefore critical steps in drug development. |
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| These types of analysis traditionally require large volumes of blood (generally 100 - 500 μl per subject per time point) to provide sufficient plasma volume for quantitative bioanalysis. Because only a limited blood volume can be drawn from each animal the number of serial samples is restricted and composite sampling must be used. This use results in lower quality PK data and an increase in the number of animals required. The large volumes also make it difficult to conduct studies in juvenile subjects. |
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| Additionally, plasma needs to be isolated from whole blood and prepared for bioanalysis using solid phase extraction, liquid-liquid extraction or protein precipitation. This time-consuming process limits throughput and consequently how many samples can be tested. |
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| Finally, there are practical challenges with shipping and storing blood samples that require controlled handling and frozen transportation and storage. |
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| The dried blood spot (DBS) method is an alternative technique that overcomes these drawbacks. |
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| DBS microvolume sampling using specialized Whatman media from GE Healthcare has been shown to be both precise and accurate for a variety of compounds from different structural classes with acceptable inter- and intra-assay variability. It is now being routinely employed in PK/TK studies. (See Supporting Documentation below for a list of some users.) The FTA® DMPK-A and FTA DMPK-B cards lyse cells and denature proteins on contact. Samples can be shipped and stored at ambient temperature and long-term stability has been demonstrated for analytes and metabolites sensitive to plasma enzymes. |
Spot. Extract. Analyze.
- Apply blood to card and let dry. Ship and store as needed at ambient temperature.
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- Punch out sample disc and elute with solvent.
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- Analyze solution by HPLC-MS/MS.
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Features and Benefits
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| Lower blood volumes |
- DBS microvolume sampling requires only 10 to 20 μl per sample.
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- Fewer experimental animals are required because Toxicokinetic data are derived from Toxicology animals, thereby eliminating satellite colonies.
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- More consistent data are obtained through more serial sampling from individual animals and less reliance on composite data.
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- Juvenile animals and humans can be studied.
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| Simpler and safer processing |
- The 3-step DBS procedure is much more straightforward than the cumbersome centrifugation, isolation and cleanup of plasma.
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- On-substrate cleanup has the potential to convey greater analyte stability, especially for enzyme-sensitive compounds.
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| Easier storage and transportation logistics |
- Room temperature stability saves on cost of dry ice shipments.
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- Shipping without dry ice allows more remote sampling, particularly important for human clinical trail samples.
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| Cost savings |
- Several of the benefits listed above, particularly reduced animal use and reduced shipping of samples on dry ice, can reduce drug development costs.
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Indicating FTA-DMPK Cards
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| These indicating cards offer the same benefits as our standard dried blood spot technology, but now for clear biosamples such as plasma, urine and cerebrospinal fluid. |
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| When a sample is applied the blue dye is displaced, leaving a lighter-colored area for easy identification. |
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| Reference: M Barfield and R Wheller (2011) Use of Dried Plasma Spots in the Determination of Pharmacokinetics in Clinical Studies: Validation of a Quantitative Bioanalytical Method. Analytical Chemistry 83 (1), 118-124. |
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| Publications |
| Please see "Published references and articles for DMPK.pdf" in Supporting Documentation list below. |
Contact information
For more information please contact your local sales representative
or email dmpk@ge.com.
| Ordering Information - Whatman FTA® DMPK Cards |
| Catalog Number |
Description |
| Whatman FTA DMPK Cards, white, for blood or other samples |
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| WB129241 |
FTA DMPK-A Cards, 100/pk |
| WB129242 |
FTA DMPK-B Cards, 100/pk |
| WB129243 |
FTA DMPK-C Cards, 100/pk |
| WB129248 |
FTA DMPK Starter Pack, 45/pk (15 x A, B and C), 3mm Uni-Core Punch and small cutting mat |
| Whatman Indicating FTA DMPK Cards, blue, for colorless samples |
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| WB120222 |
FTA DMPK-A IND Cards, 100/pk |
| WB120223 |
FTA DMPK-B IND Cards, 100/pk |
| WB120224 |
FTA DMPK-C IND Cards, 100/pk |
| WB120225 |
FTA DMPK IND Starter Pack, 45/pk (15 x A, B and C), 3mm Uni-Core Punch and small cutting mat |
| Punching and Drying Accessories |
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| WB100039 |
Harris Uni-Core Punch, 3mm, 4/pk with two 2.5" x 3" cutting mats |
| WB100040 |
Harris Uni-Core Punch, 6mm, 4/pk with two 2.5" x 3"cutting mats |
| WB100038 |
Harris Micro Punch, 3mm, 1/pk with one 5.9" x 7.9" cutting mat |
| WB100042 |
Replacement Tip for Micro Punch, 3mm, 1/pk |
| WB100020 |
Cutting Mat, large, 5.9" x 7.9" |
| 10537173 |
Dry Rak (without Velcro), holds 11 cards, 10/pk |
| 10539521 |
Dry Rak (with Velcro), holds 11 cards, 10/pk |
| Storage and Shipping Accessories |
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| 10548232 |
Plastic Ziploc Storage Bags, 4"x6", 100/pk |
| 10534321 |
Foil Barrier Ziploc Bags, for cards 95 x 130 mm max, 100/pk |
| WB100003 |
Desiccant Packets, Indicating, 1g, 1000/pk |
| 10548236 |
Glassine Envelopes, 3.25" x 4.88", 100/pk |
| 10534150 |
Biohazard Labels, 0.88" x 0.88", 1000/pk |
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